|
KMID : 0043320060290090757
|
|
Archives of Pharmacal Research
2006 Volume.29 No. 9 p.757 ~ p.761
|
|
|
Protoberberine Alkaloids and their Reversal Activity of P-gp Expressed Multidrug Resistance (MDR) from the Rhizome of Coptis japonica Makino
|
|
Min Yong-Deuk
Yang Min-Cheol
Lee Kyu-Ha
Kim Kyung-Ran
Choi Sang-Un
Lee Kang-Ro
|
|
|
Abstract
|
|
|
|
Six protoberberine alkaloids were isolated from the chloroform layer of the rhizome of Coptis japonica Makino (Ranunculaceae). The structures of the isolated compounds were determined to be 6-([1,3]dioxolo[4,5-g]isoquinoline-5-carbonyl)-2,3-dimethoxy-benzoic acid methyl ester (1), oxyberberine (2), 8-oxo-epiberberine (3), 8-oxocoptisine (4), berberine (5) and palmatine (6) by physicochemical and spectroscopic methods. The compound 3 (8-oxo-epiberberine) was first isolated from natural sources. The compounds were tested for cytotoxicity against five tumor cell lines in vitro by SRB method, and also tested for the MDR reversal activities. Compound 4 was of significant P-gp MDR inhibition activity with ED50 value 0.018 μg/mL in MES-SA/DX5 cell and 0.0005 μg/mL in HCT15 cell, respectively.
|
|
|
KEYWORD
|
|
|
Coptis japonica, Ranunculaceae, Protoberberine alkaloid, 8-Oxo-epiberberine, Multidrug resistance
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|